Abstract. Scaling behaviour in nonstationary time series can be successfully detected using the detrended fluctuation analysis (DFA). Observational time series often do not show a stable and uniform scaling behaviour, given by the presence of a unique clear scaling region. The deviations from uniform power-law scaling, which suggest the presence of changing dynamics in the system under study, can be identified and quantified using an appropriate instability index. In this framework, the scaling behaviour of the 1981–2007 seismicity in Umbria-Marche (central Italy), which is one of the most seismically active areas in Italy, was investigated. Significant deviations from uniform power-law scaling in the seismic temporal fluctuations were revealed mostly linked with the occurrence of rather large earthquakes or seismic clusters.